- Find MSDS or SDS, a COA, data sheets and more information. AFDye™ 647 Maleimide is a water-soluble, far-red-fluorescent dye that is spectrally similar to other 650 dyes that are used for flow cytometry, microscopy and other applications. Molecular weight [M]– was determined by MS to be 1,110.3. This Anti-CD20 Antibody, clone FMC7, Alexa Fluor® 647 Conjugate is validated for use in Flow Cytometry for the detection of CD20. Alexa Fluor® 647 conjugates are highly photostable and remain fluorescent over a broad pH range. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated Calbindin (D1I4Q… Cited in 1 publication(s). Alexa Fluor® 647- and APC-conjugated secondary antibodies are the best choice for flow cytometry when secondary antibodies fluorescing at these wavelengths are desired. Blog Dandk September 25, 2018. Molecular Formula C 71 H 108 N 5 O 19 S 4; Average mass 1463.899 Da; Monoisotopic mass 1462.651611 Da; ChemSpider ID 27473830 - Charge - Double-bond stereo - 13 of 14 defined stereocentres. This is in sharp contrast to colloidal gold conjugates which usually are prepared by centrifugation to remove the largest aggregates, and frequently contain smaller aggregates. Cruz Marker™ Molecular Weight Standards detection antibody conjugated to Alexa Fluor ® 680 recommended for detection of Cruz Marker™ Molecular Weight Standards by Near-Infrared WB this reagent must be used in combination with Cruz Marker™ Molecular Weight Standards (sc-2035), available only from Santa Cruz Biotechnology, Inc. For stable signal generation in imaging and flow cytometry, Super Fluor 647 dye is pH-insensitive over a wide molar range.Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Mouse Monoclonal Anti-CD45 Antibody (HI30) [Alexa Fluor® 647]. The theoretical molecular weight for most of our CD45 antibodies is 147 kDa based off the first isoform. Tested Reactivity: Human, Primate. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, ... [Alexa Fluor® 647] and receive a gift card or discount. Super Fluor 647 dye is a bright, far-red–fluorescent dye with excitation ideally suited for the 594 nm or 633 nm laser lines. 1-858-677-9432. sales@axispharm.com. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. Detection of LC3B in HeLa Human Cell Line by Flow Cytometry. They are brighter than Cy5 and DyLight 650 in aqueous mounting media. More details: Systematic name ... Alexa Fluor 647-cas tasteron. Anti-Iba1/AIF1 Antibody, clone 20A12.1, Alexa Fluor® 647 Conjugate is an antibody against AIF1 for use in Immunofluorescence. Invitrogen™ Dextran, Alexa Fluor™ 647; 10,000 MW, Anionic, Fixable Rigorous methods for removing as much unconjugated dye as practical, followed by assay of dextran conjugates by thin-layer chromatography to help ensure absence of low molecular weight contaminants Rabbit recombinant monoclonal JAK2 (phospho Y1007 + Y1008) antibody [E132] conjugated to Alexa Fluor® 647. Labeled Tubulin-Alexa Fluor® 647 will polymerize into microtubules when supplemented with guanosine-5'-triphosphate (GTP), warmed to 37˚C, and kept above its critical concentration. Materials Kit Contents Catalog Number 064705 Purity >99%. Bioinformatics. Labeled Tubulin-Alexa Fluor® 647 $ 295.00. AP Fluor 647 Picolyl Azide (Alexa Fluor 647 Picolyl Azide analog) is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the e. ADC Technologies & Analytical Services & Worldwide Leading PEG Supplier. A significant advantage to using long wavelength dyes such as Cy5 or AFDye™ 647 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum. ... Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC). HeLa human cervical epithelial carcinoma cell line either treated with 50 µM chloroquine for 24 hours (filled histogram) or untreated (open histogram) was stained with Rabbit Anti-Human LC3B Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # IC9390R). Polymerization activity is detectable in a variety of experimental systems including fluorescence microscopy assays, turbidity assays, and GTPase assays. QUANTITY: PRICE: 0.1 mg: $295: 5 x 0.1 mg: $1,475 $995: 10 x 0.1 mg: $2,950 $1,795: Other Quantity / International Shipping: Request a Quote . It is a member of the CD28 family and is highly expressed on activated T cells. Product Description. Alexa Fluor 647-castasteron. - Find MSDS or SDS, a COA, data sheets and more information. Alexa Fluor® 647-conjugated antibodies absorb light maximally around 651 nm and fluoresce maximally around 667 nm. The Alexa®, or Alexa Fluor®, dyes were introduced by Molecular Probes, Inc. (now part of Life Technologies, Inc.). Molecular Masses. ICOS is a homodimeric type I transmembrane glycoprotein with an approximate molecular weight of 50-60 kDa. Alexa Fluor 647 (AF647) is a fluorescence dye with a peak excitation of 650 nm, a peak emission maximum of 665 nm, and an extinction coefficient of 203,000 cm(–1) M(–1) (3). Anti-VEGF Antibody, Alexa Fluor® 647 Conjugate CAS - Find MSDS or SDS, a COA, data sheets and more information. Conjugates produced with the bright and photostable Alexa Fluor 647 dye have absorption and fluores- However, APC tends to be brighter while Alexa Fluor® 647 is more optimal for intracellular applications. Cruz Marker™ Molecular Weight Standards detection antibody conjugated to Alexa Fluor ® 647 recommended for detection of Cruz Marker™ Molecular Weight Standards by RGB WB this reagent must be used in combination with Cruz Marker™ Molecular Weight Standards (sc-2035), available only from Santa Cruz Biotechnology, Inc. The excitation and emission maxima are nearly identical to those of APC. Unlike Cy5 dye, the Alexa Fluor 647 dye is resistant to quench-ing at high degrees of labeling.1 Each of the vials of Alexa Fluor 647 succinimidyl ester supplied in the kit is sufficient for labeling one sample (20–100 µg at a concentration of 1 mg/ml) of protein that has a molecular weight between ~12 and ~150 kDa. Afdye 647 Azide Click Chemistry Tools In vitro Protein Labeling: Reaction of SNAP-Surface Alexa Fluor® 647 (10 µM) with purified SNAP-tag protein (5 µM) in vitro, followed by mass spec analysis, indicated an efficiency of labeling of 90%. Afdye 647 azide click chemistry tools alexa fluor 647 picolyl azide afdye 647 azide click chemistry tools calfluor 647 azide click chemistry tools. Molecular weight [M]– was determined by MS to be 1109.3. Validated: WB, Flow, Flow-CS, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P. 100% Guaranteed. face Alexa Fluor 647 was determined to be 99% by HPLC analysis. Properties and structures A range of Alexa dyes is available, with emission wavelengths spanning the visible spectrum and extending into the infrared region of the spectrum. Alexa Fluor® 647-conjugated antibodies absorb light maximally around 651 nm and fluoresce maximally around 667 nm. They are brighter than Cy5 and DyLight 650 in aqueous mounting media. Facebook Twitter linkedin. Alexa Fluor® 647- and APC-conjugated secondary antibodies are the best choice for flow cytometry when secondary antibodies fluorescing at these wavelengths are desired. The molecular mass (MM) of AlexaFlour 647 is listed in the table as 1155.06 g/mol, with a reference to .However, this article says "The predicted molecular weight for the Alexa647 peptide is 1155.06, obs = 1155.0".Here, "Alexa647 peptide" is referencing a GGGK peptide conjugate of Alexa647. For dextran internalization, cells were incubated with 10 µg/ml dextran–Alexa Fluor 488 or dextran–Alexa Fluor 647 (3,000 molecular weight; Thermo Fisher Scientific) … Apoptosis is an essential biological process that maintains homeostasis of tissues and organs in concert with proliferation, growth, and differentiation (4-6). TCAPQ647 is a small permeation peptide that comprises QSY21, a broad-spectrum quencher, and Alexa Fluor 647, a near-infrared fluorophore. - Find MSDS or SDS, a COA, data sheets and more information. This Cell Signaling Technology antibody is conjugated to Alexa Fluor ® 647 fluorescent dye and tested in-house for direct immunofluorescent analysis in rat cells. In vitro Protein Labeling: Reaction of SNAP-Surface Alexa Fluor 647 (10 µM) with purified SNAP-tag protein (5 µM) in vitro, followed by mass spec analysis, indicated an efficiency of labeling of 90%. AFDye™ 647 conjugates ideally suited for the 633 nm or 647 nm laser lines. AFDye™ 647 (Alexa Fluor ® 647 analog) is a water-soluble, bright, far-red-fluorescent dye with excitation ideally suited for the 633 nm or 647 nm laser lines. Validated in Flow Cyt, ICC/IF and tested in Human. Search results for Alexa fluor 647 anti-mouse antibody at Sigma-Aldrich Immunocytochemistry/ Immunofluorescence - Anti-NDRG1 antibody [EPR5593] (Alexa Fluor® 647) (ab199471) ab199471 staining NDRG1 in HeLa cells. Submit a Review. Anti-Puromycin, clone 17H1, Alexa Fluor® 647 Conjugate Antibody This Anti-Puromycin, clone 17H1 is validated for use in Immunocytochemistry for the detection of Puromycin. face Alexa Fluor 647 was determined to be 99% by HPLC analysis. Alexa Fluor 647 Azide Molecular Weight. Ac-rkkrrorrrGK(QSY21)DEVDAPC(Alexa Fluor 647)-NH2 (TCAPQ647) is a membrane-permeable, activatable caspase substrate developed for optical imaging of programmed cell death (apoptosis) (1, 2). Alexa Fluor®* 647 FLUORONANOGOLD ... no aggregates or other molecular weight impurities. Alexa Fluorfi 647 Protein Labeling Kit (A20173) Introduction Molecular Probes’ Alexa Fluor ® 647 Protein Labeling Kit provides a convenient means to label proteins with our superior Alexa Fluor 647 dye. Molecular weight for most of our CD45 antibodies is 147 kDa based the! Of the CD28 family and is highly expressed on activated T cells 647 nm laser lines other... 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2020 alexa fluor 647 molecular weight